讲座题目：Chemical Sensing Molecules for Bioanalysis
主讲人：Koji Suzuki 教授，Keio University
主持人：田阳 教授
开始时间：2016年6月24日（星期五）下午15：30
讲座地址：闵行校区实验D楼207
主办单位：Betway必威西汉姆联
报告人简介：
Koji Suzuki, Professor of Department of Applied Chemistry, Faculty of Science and Technology, Keio University, was born in 1954 in Tokyo. He received Bachelor (1977), Master (1979) and PhD. (1982) degrees of Engineering from Keio University. He became a faculty member of Keio University in 1982, Associate Professor in 1993, and full Professor in 1998. From 1990 − 1992, he was a Guest Professor at the Swiss Federal Institute of Technology (ETH), Zurich. He is now the President of the Japan Society for Analytical Chemistry (JSAC), the Chair of Analytical Division of the 116th Committee for Chemistry of Functional Organic Chemicals at The Japan Society for the Promotion of Science (JSPS), and the Cooperative Member of the Science Council of Japan (SCJ, Cabinet Office) and the Chair of Analytical Chemistry Division of SCJ. He received many awards including The Chemical Society of Japan Award for Creative Work in 1997, The Hitachi Environment Foundation Environment Prize in 2005, The JSAC Award in 2007, and The JASC-JAIMA Advanced Analytical Technology Award in 2009. His research focuses on chemical and biochemical sensors based on functional molecule creation.
报告摘要：
The creation of original chemical sensors is one of the main concerns in our research group. In biological research fields, chemical sensors such as synthetic biochemical probes play an important role in the investigation of biological functions, and in cellular or in vivo imaging.
One of our recent research highlights was the creation of a magnesium-imaging probe (KMG series fluorescent molecules) for living cells. The KMG-series molecules for magnesium (Mg) imaging were designed and synthesized based on our prior knowledge of Mg ionophore design. While the first example (KMG-20) was based on a coumarin fluorophore, we later developed the fluorescein derivative KMG-104, which is the best magnesium fluorescent probe for imaging applications in the cytoplasm of living cells and also developed KCM-1 for simultaneous imaging of Mg and Ca ions in living cells. More recently, a flash-type Mg fluorescent probe was developed for specific protein labeling.
Several bioluminescent (BL) systems have been investigating based on synthetic coelenterazine (CTZ) derivatives as substrates in combination with Renilla luciferase (Rluc) variants or artificial luciferases (Aluc) as the enzyme. It was found that extending the conjugated system at the 6-carbon position of CTZ is more effective compared to extensions at the 2-, 5-, or 8-carbon positions. The 6-position carbon variants of CTZ combined with the known Rluc mutant Rluc8.6 resulted in the most intense bioluminescence in the blue spectral region. In addition, with the system consisting of a CTZ derivative and the artificial luciferase Aluc, we have succeeded in the development of the most high-intensity artificial bioluminescence system.