讲座题目：New electrochemical tools to monitor neurotransmitter release
主讲人：Ann-Sofie Cans 副教授，Chalmers University of Technology
主持人：田阳 教授
开始时间：2016年5月3日（星期二）上午10:00
讲座地址：闵行校区实验D楼207
主办单位：Betway必威西汉姆联
报告人简介：
Ann-Sofie Cans, Department of Chemistry and Chemical Engineering Chalmers University of Technology. Ann-Sofie Cans is currently an Associate professor of Analytical Chemistry at the Department of Chemistry and Chemical Engineering, at the Chalmers University of Technology, Sweden. She has been working with development of cell models to mimic cell function and bioanalytical techniques for applications in neuroscience and membrane biophysics. This involves techniques such as electrochemistry, electrochemical biosensors, electrochemical quartz crystal microbalance with dissipation, and micromanipulation techniques for cells and model membranes. She obtained her Ph.D. at Gothenburg University in 2003 and then pursued her post-doctoral training in the Department of Chemistry at Penn State University, USA (2004-2007) working in the different labs of Professor Paul Weiss, Professor Anne Andrews and Professor Christine Keating. She started her academic position at Chalmers University of Technology in Sweden (2007).
报告摘要：
Electrochemical methods are powerful techniques for monitoring release of electroactive species released from secretory cells. Depending on the type of method used, quantitative and qualitative information of vesicle content release at cells can be recorded with high temporal resolution. However, the spatial resolution of electrochemical sensors are often low and the analytes that can be detected are limited to molecules that are electroactive.
New electrochemical based methods have been developed to increase the spatial resolution of electrochemical imaging of neurotransmitter release at secretory cells. We here show that microfabrication of a lithographic movable thin film 16-microelectrode array (MEA) probe, provides the ability to place the probe in close proximity to the surface of adherent single cells and recording of single exocytotic release events. Analysis and modelling of the amperometric spikes from single vesicle content release by co-detected from multiple electrodes provides a window at the probe surface that can be used for electrochemical imaging of exocytotic release with high spatio-temporal resolution.
Many neurotransmitters important in brain function, such as glutamate and acetylcholine are not electroactive, and therefore are more difficult to study. Electrochemical sensors constructed with enzymes have been developed and is based on an enzymatic reaction to convert non-electroactive analytes to an electroactive product that is detectable by an electrode surface. However, even though these type of sensors have very high sensitivity, the response time for these sensors are often relatively slow compared to the kinetics for neurochemical fluctuations that occur during exocytotic activity at cells, which occur on the milliseconds time scale. In a new approach for construction of enzyme based electrochemical sensors, we show that the temporal resolution of these probes can be significantly improved down to the time scales of how fast neurotransmitter release actually occurs.